Cytotoxicity of Silorane and Methacrylate based Dental Composites on Human Pulp Cells

Prashanthi S Madhyastha; Kumar M.R Bhat; Divya Padma; Madhu Keshava Bangera; Dilip G Naik; N. Srikant; Ravindra Kotian

doi:10.4103/jofs.jofs_312_20

ORIGINAL ARTICLE

Year : 2021 | Volume : 13 | Issue : 1 | Page : 13-18

Cytotoxicity of Silorane and Methacrylate based Dental Composites on Human Pulp Cells

Prashanthi S Madhyastha¹, Kumar M.R Bhat², Divya Padma³, Madhu Keshava Bangera¹, Dilip G Naik⁴, N. Srikant⁵, Ravindra Kotian¹
¹ Department of Dental Materials, Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, India
² Department of Anatomy, Ras Al Khaimah College of Medical Sciences, RAK Medical & Health Sciences University, Ras Al Khaimah, UAE
³ Manipal GoK Bio Incubator, School of Life Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India
⁴ Department of Periodontics, Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, India
⁵ Department of Oral Pathology and Microbiology, Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, India

Correspondence Address:
Madhu Keshava Bangera
Department of Dental Materials, Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal 575001, Karnataka
India

Source of Support: None, Conflict of Interest: None

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DOI: 10.4103/jofs.jofs_312_20

Introduction: The ingredients from the dental restoratives are known to leach and elicit a host response. The prerequisite to deem a material biocompatible requires its toxicologic evaluation. The study was performed to analyze the probable toxicity resulting from silorane-based composite (SBC) with methacrylate-based composite (MBC). Materials and Methods: The in vitro cytotoxicity test, methyl thiazolyl tetrazolium (MTT) assay, evaluated the cell viability and proliferation rate of dental pulp cells (DPCs). The extirpated pulp was cultured in α-MEM-containing supplements and incubated at 37°C. DPCs were subjected to varying doses of SBC or MBC at different time intervals after attaining confluence and monitored for proliferation and viability via MTT assay. An independent Student t test was performed to compare the effect of composites on the DPC. The cytotoxicity levels were compared using one-way analysis of variance and posthoc Tukey multiple comparison test at 5% level of significance and P-value of <0.05. Results: DPC exposed to MBC showed higher viability than SBC. The MTT assay reported the number of viable cells as (>90%) in the first 24 hours. The count significantly reduced by the end of 48 hours (minimum 65% in 25 μg/ml) at all concentrations (P < 0.05). SBC had lower survival than MBC in all concentrations and periods. Except at 5 μg/ml concentration at 48 hours in SBC, no statistically significant values were reported. Conclusion: DPCs are prone to the cytotoxicity caused by dental composite. In contrast to MBC, the cytotoxicity of SBC declines overtime.

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