Journal of Orofacial Sciences

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 12  |  Issue : 1  |  Page : 52--60

Predicting the Malignant Transformation of Oral Submucous Fibrosis Using Quantitative Biomarkers p63 and CD31


Radhika Manoj Bavle1, Konda Paremala2, Makarala Sowmya1, Muniswamappa Sudhakara1, Venugopal Reshma1, Sreelatha Hosthor1 
1 Department of Oral and Maxillofacial Pathology, Krishnadevaraya College of Dental Sciences & Hospital, Bangalore, Karnataka, India
2 Department of Oral Pathology, Govt Dental College and Hospital, Afzalgunj, Hyderabad, Telangana, India

Correspondence Address:
Dr Makarala Sowmya
Department of Oral Pathology, Krishnadevaraya College of Dental Sciences, MVIT Campus, Via Yelahanka, Hunusamaranahalli, Bangalore-562157, Karnataka
India

Introduction: Oral submucous fibrosis (OSMF) is a pre-malignant condition highly prevalent in India with a malignant transformation rate of 2–8%. Its incidence amongst younger population has risen due to increased consumption of commercial preparations of areca. p63, a homolog of p53, has a role in epithelial proliferation and is frequently altered in dysplasia and associated with tumorigenesis. CD31 is a highly specific endothelial marker with varied expression in epithelial dysplasia and carcinoma. Epithelial proliferation and underlying angiogenic support are vital processes for malignant transformation. Therefore the present study aims to determine if p63 and CD31 expression is associated with increased malignant transformation of OSMF. Materials and Methods: A cross-sectional study wherein n = 36 OSMF samples were histologically graded into Group I (Early-Ea), II (Moderately advanced-MA) and III (Advanced-Ad) and analysed for anti-p63 and anti-CD31 antibodies. The expression was evaluated quantitatively and by pattern of distribution across the groups and statistically analysed with Chi-Square and Kruskal-Wallis tests (SPSS v2.0). Results: p63 expression of epithelium in Group I ∼100% (basal, suprabasal), II − 60.9% (basal, spinous), III − 62.5% (basal, spinous, granular) with a significant P-value < 0.001*. Predominant pattern of CD31 positive vessels in Group I ∼60% (constricted), II > 50% (normal diameter) and III ∼ 75% (dilated) with a significant P-value of 0.02*. Conclusion: A significant linear increase in nuclear staining of p63 and involvement of epithelial strata observed from Ea → MA → Ad grade of OSMF. CD31 expression exhibited more dilated vessels as OSMF grade increased from Ea → Ad. Therefore p63 and CD31 could be used as quantitative predictive biomarkers of malignant transformation of OSMF.


How to cite this article:
Bavle RM, Paremala K, Sowmya M, Sudhakara M, Reshma V, Hosthor S. Predicting the Malignant Transformation of Oral Submucous Fibrosis Using Quantitative Biomarkers p63 and CD31.J Orofac Sci 2020;12:52-60


How to cite this URL:
Bavle RM, Paremala K, Sowmya M, Sudhakara M, Reshma V, Hosthor S. Predicting the Malignant Transformation of Oral Submucous Fibrosis Using Quantitative Biomarkers p63 and CD31. J Orofac Sci [serial online] 2020 [cited 2020 Nov 30 ];12:52-60
Available from: https://www.jofs.in/article.asp?issn=0975-8844;year=2020;volume=12;issue=1;spage=52;epage=60;aulast=Bavle;type=0