Journal of Orofacial Sciences

ORIGINAL ARTICLE
Year
: 2021  |  Volume : 13  |  Issue : 1  |  Page : 13--18

Cytotoxicity of Silorane and Methacrylate based Dental Composites on Human Pulp Cells


Prashanthi S Madhyastha1, Kumar M.R Bhat2, Divya Padma3, Madhu Keshava Bangera1, Dilip G Naik4, N. Srikant5, Ravindra Kotian1 
1 Department of Dental Materials, Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, India
2 Department of Anatomy, Ras Al Khaimah College of Medical Sciences, RAK Medical & Health Sciences University, Ras Al Khaimah, UAE
3 Manipal GoK Bio Incubator, School of Life Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India
4 Department of Periodontics, Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, India
5 Department of Oral Pathology and Microbiology, Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, India

Correspondence Address:
Madhu Keshava Bangera
Department of Dental Materials, Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal 575001, Karnataka
India

Introduction: The ingredients from the dental restoratives are known to leach and elicit a host response. The prerequisite to deem a material biocompatible requires its toxicologic evaluation. The study was performed to analyze the probable toxicity resulting from silorane-based composite (SBC) with methacrylate-based composite (MBC). Materials and Methods: The in vitro cytotoxicity test, methyl thiazolyl tetrazolium (MTT) assay, evaluated the cell viability and proliferation rate of dental pulp cells (DPCs). The extirpated pulp was cultured in α-MEM-containing supplements and incubated at 37°C. DPCs were subjected to varying doses of SBC or MBC at different time intervals after attaining confluence and monitored for proliferation and viability via MTT assay. An independent Student t test was performed to compare the effect of composites on the DPC. The cytotoxicity levels were compared using one-way analysis of variance and posthoc Tukey multiple comparison test at 5% level of significance and P-value of <0.05. Results: DPC exposed to MBC showed higher viability than SBC. The MTT assay reported the number of viable cells as (>90%) in the first 24 hours. The count significantly reduced by the end of 48 hours (minimum 65% in 25 μg/ml) at all concentrations (P < 0.05). SBC had lower survival than MBC in all concentrations and periods. Except at 5 μg/ml concentration at 48 hours in SBC, no statistically significant values were reported. Conclusion: DPCs are prone to the cytotoxicity caused by dental composite. In contrast to MBC, the cytotoxicity of SBC declines overtime.


How to cite this article:
Madhyastha PS, Bhat KM, Padma D, Bangera MK, Naik DG, Srikant N, Kotian R. Cytotoxicity of Silorane and Methacrylate based Dental Composites on Human Pulp Cells.J Orofac Sci 2021;13:13-18


How to cite this URL:
Madhyastha PS, Bhat KM, Padma D, Bangera MK, Naik DG, Srikant N, Kotian R. Cytotoxicity of Silorane and Methacrylate based Dental Composites on Human Pulp Cells. J Orofac Sci [serial online] 2021 [cited 2021 Sep 17 ];13:13-18
Available from: https://www.jofs.in/article.asp?issn=0975-8844;year=2021;volume=13;issue=1;spage=13;epage=18;aulast=Madhyastha;type=0