ORIGINAL ARTICLE
Year : 2022 | Volume
: 14 | Issue : 2 | Page : 88--92
Antibacterial Activity of Robusta Coffee (Coffea robusta) Husk Extract Against Streptococcus mutans and Lactobacillus acidophilus: In Vitro Study
Arlin R Kusumawardani1, Andi M Machbub1, Rendra C Prasetya2, Nadie Fatimatuzzahro2, Tantin Ermawati2
1 Faculty of Dentistry, Jember University, Jember, Indonesia
2 Department of Biomedic, Faculty of Dentistry, Jember University, Jember, Indonesia
Correspondence Address:
Nadie Fatimatuzzahro
Faculty of Dentistry, Jember University, Kalimantan No 37, Kampus Tegalboto, Jember, Jawa Timur 68121
Indonesia
Introduction: Caries is a tooth and oral illness caused by Streptococcus mutans and Lactobacillus acidophilus bacteria. The growth of caries-causing bacteria can be controlled by using 0.2% chlorhexidine mouthwash to control plaque, however long-term usage of 0.2% chlorhexidine causes negative effects. Natural components such as robusta coffee (Coffea robusta) husk, which possesses active compounds of polyphenols, flavonoids, alkaloids, tannins, and saponins as alternative antibacterials, can be used to reduce adverse effects. The aim of this research is to assess the antibacterial activity of robusta coffee husk extract against S. mutans and L. acidophilus. Materials and Methods: The robusta coffee husk was extracted using the maceration process with 96% ethanol as the solvent. Antibacterial test is conducted against S. mutans and L. acidophilus using disc diffusion method (Kirby-Baurer) with six treatment groups of extract concentration 250, 500, 750, 1000 mg/mL, positive control (0.2% chlorhexidine), and negative control (aquades steril). The zone of inhibition was measured in millimetres using a digital calliper (mm). The SPSS application was used to examine the calculation findings, which included the Shapiro-Wilk, Levene, One Way ANOVA, and Post Hoc LSD tests. Results: Robusta coffee husk extract at 250, 500, 750, and 1000 mg/mL doses shown bactericidal activity in S. mutans (radical zone) and bacteriostatic activity in L. acidophilus (irradical zone). Conclusion: Robusta coffee husk extract has an antibacterial activity against S. mutans and L. acidophilus. The highest inhibition zone was demonstrated by the 1000 mg/mL concentration of extract.
How to cite this article:
Kusumawardani AR, Machbub AM, Prasetya RC, Fatimatuzzahro N, Ermawati T. Antibacterial Activity of Robusta Coffee (Coffea robusta) Husk Extract Against Streptococcus mutans and Lactobacillus acidophilus: In Vitro Study.J Orofac Sci 2022;14:88-92
|
How to cite this URL:
Kusumawardani AR, Machbub AM, Prasetya RC, Fatimatuzzahro N, Ermawati T. Antibacterial Activity of Robusta Coffee (Coffea robusta) Husk Extract Against Streptococcus mutans and Lactobacillus acidophilus: In Vitro Study. J Orofac Sci [serial online] 2022 [cited 2023 Mar 28 ];14:88-92
Available from: https://www.jofs.in/article.asp?issn=0975-8844;year=2022;volume=14;issue=2;spage=88;epage=92;aulast=Kusumawardani;type=0 |
|
